#抗擊疫情,我們在行動#?據國家及各地衛健委每日信息發布統計,截止2020.4.5日,國內累計確診新型冠狀感染肺炎82930例,累計治愈77237例,現有確診2355例,目前已有316市實現現有確診病例清零,國家衛健委聯防聯控辦公室表示:我國本輪疫情流行高峰已經過去,新增發病數在持續下降,疫情總體保持在較低水平,外防輸入已成為我國疫情防控的重中之重。面對新冠肺炎疫情,在我國及去全球現行的醫療技術體系之下,在病毒樣本提取中,核酸提取檢測與CT檢測成為COVID-19冠狀病毒的重要檢測手段。全自動核酸提取儀是應用配套的核酸提取試劑來使得樣本核酸提取工作自動完成的儀器,是每個實驗室與生物分子相關的分離純化工作必不可少的儀器,核酸提取儀分為兩類,一類是大型自動化實驗室實驗儀器,一般稱為自動液體工作站;另一類是小型自動核酸提取儀,利用封裝好的配套試劑自動完成提取純化過程。
以全(quan)自(zi)動核(he)(he)酸提取儀的(de)(de)工作(zuo)原理(li)為例,在(zai)自(zi)動核(he)(he)酸提取系統工作(zuo)時,會(hui)有(you)氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)污染產生。世界衛生組織報告(gao)曾(ceng)指出,病毒(du)或細菌可(ke)以通(tong)(tong)過氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)經長距離傳(chuan)播(bo)(bo)而在(zai)短(duan)期(qi)內導致大面積(ji)感染,回看過往,業內人(ren)(ren)士指出研究人(ren)(ren)員(yuan)發現(xian)SARS病毒(du)通(tong)(tong)過空氣(qi)氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)路(lu)徑傳(chuan)播(bo)(bo)有(you)明(ming)確的(de)(de)證(zheng)據。其次,H5N1禽流感病毒(du)也存在(zai)通(tong)(tong)過氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)路(lu)徑傳(chuan)播(bo)(bo)的(de)(de)證(zheng)據。鑒于新(xin)冠病毒(du)與(yu)SARS具有(you)80%的(de)(de)基因序(xu)列相似度,推測(ce)新(xin)冠病毒(du)與(yu)SARS的(de)(de)物理(li)性(xing)質可(ke)能(neng)類(lei)似,不排除通(tong)(tong)過氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)傳(chuan)播(bo)(bo)的(de)(de)可(ke)能(neng)。在(zai)COVID-19冠狀病毒(du)核(he)(he)酸的(de)(de)檢(jian)測(ce)當(dang)中,存在(zai)于實(shi)驗室桌面、儀器、耗材以及空氣(qi)中的(de)(de)DNA污染,對于檢(jian)測(ce)實(shi)驗會(hui)產生嚴重的(de)(de)干(gan)擾,尤其是氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)攜帶的(de)(de)DNA分子(一個氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)能(neng)攜帶上萬個DNA),在(zai)空氣(qi)中漂浮,難以消(xiao)除。PCR擴增過程(cheng)中會(hui)產生DNA/RNA氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)污染的(de)(de)問題(ti)。極其微量的(de)(de)氣(qi)溶(rong)(rong)(rong)(rong)膠(jiao)(jiao)污染即(ji)可(ke)造成假陽性(xing)的(de)(de)結果,進而可(ke)能(neng)引(yin)起(qi)整個PCR實(shi)驗室的(de)(de)污染,造成高概率的(de)(de)新(xin)冠肺炎核(he)(he)酸檢(jian)測(ce)假陰(yin)性(xing)事(shi)件,嚴重的(de)(de)甚至(zhi)要關閉(bi)實(shi)驗室。
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目前治理氣(qi)(qi)溶(rong)(rong)膠污(wu)(wu)(wu)(wu)染(ran)(ran)的(de)常(chang)(chang)規(gui)方(fang)法有:酒(jiu)精擦拭、紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)照射(she)、高壓(ya)變性(xing)。祛除DNA污(wu)(wu)(wu)(wu)染(ran)(ran)可以選(xuan)擇紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)燈殺菌(jun)(jun),相對過濾吸(xi)附法,紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)照射(she)屬于(yu)更高效(xiao)的(de)消殺方(fang)式,常(chang)(chang)規(gui)紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)照射(she)建議根(gen)據容(rong)器(qi)體積,或者(zhe)空間(jian)體積,來選(xuan)擇合適的(de)紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)燈。值得注意的(de)是(shi),要(yao)祛除DNA污(wu)(wu)(wu)(wu)染(ran)(ran)而不(bu)是(shi)僅僅滅菌(jun)(jun)的(de)話,常(chang)(chang)規(gui)紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)照射(she)建議應延長至2小(xiao)時,才能(neng)有效(xiao)滅殺空間(jian)內的(de)病毒性(xing)氣(qi)(qi)溶(rong)(rong)膠及細菌(jun)(jun)。如杭州某企業研(yan)制(zhi)的(de)全(quan)(quan)自動核酸提(ti)取(qu)儀,著重在其儀器(qi)內部配置了一款(kuan)高效(xiao)的(de)紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)燈,利用UV滅菌(jun)(jun)防污(wu)(wu)(wu)(wu)染(ran)(ran)控(kong)制(zhi),紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)燈工作(zuo)時配合一次性(xing)耗材,進一步嚴格控(kong)制(zhi)孔(kong)間(jian)污(wu)(wu)(wu)(wu)染(ran)(ran)及批(pi)次間(jian)污(wu)(wu)(wu)(wu)染(ran)(ran),有效(xiao)預防交叉污(wu)(wu)(wu)(wu)染(ran)(ran)。該紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)燈由朗普科技研(yan)發(fa),產品(pin)型號為DC12V/24V的(de)低(di)壓(ya)安(an)全(quan)(quan)直流(liu)紫(zi)(zi)(zi)(zi)(zi)外(wai)線(xian)(xian)燈,具有抗(kang)電磁干(gan)擾、啟(qi)動沖(chong)擊電流(liu)小(xiao)、頻繁開關不(bu)易黑頭(tou)、光(guang)衰小(xiao)、故障率(lv)低(di)等優點,非常(chang)(chang)適用于(yu)核算(suan)提(ti)取(qu)儀中(zhong)做好良(liang)好的(de)滅菌(jun)(jun)裝置。
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DC12V/24V低(di)壓(ya)(ya)直流紫外線(xian)(xian)燈(deng)由(you)朗普(pu)(pu)(pu)科技自主研發,根據(ju)當前疫(yi)情(qing)(qing)防(fang)(fang)控的形勢及(ji)接下(xia)來(lai)對于生物安全防(fang)(fang)護(hu)及(ji)空間消(xiao)(xiao)毒防(fang)(fang)護(hu)措施的需要,有效適用于精密(mi)消(xiao)(xiao)毒檢(jian)測(ce)(ce)(ce)(ce)儀(yi)器(qi)、水質(zhi)分(fen)析(xi)儀(yi)器(qi)、血液病毒滅活檢(jian)測(ce)(ce)(ce)(ce)儀(yi)、智(zhi)能機器(qi)人及(ji)售賣(mai)機、快遞外賣(mai)餐箱(xiang)等領域。DC12V/24V低(di)壓(ya)(ya)直流紫外線(xian)(xian)燈(deng)擁有朗普(pu)(pu)(pu)紫外線(xian)(xian)快速選(xuan)型發明專(zhuan)(zhuan)(zhuan)利,單燈(deng)紫外線(xian)(xian)殺(sha)菌強度高(gao),殺(sha)菌用時短;同時獲得國家實用新型專(zhuan)(zhuan)(zhuan)利證(zheng)書及(ji)紫外線(xian)(xian)專(zhuan)(zhuan)(zhuan)用鎮流CE認證(zheng)及(ji)殺(sha)菌燈(deng)CE、ROHS雙認證(zheng);通過廣東省微生物分(fen)析(xi)測(ce)(ce)(ce)(ce)驗中心分(fen)析(xi)報(bao)告,使用DC12V/24V低(di)壓(ya)(ya)直流紫外線(xian)(xian)燈(deng)對特定(ding)檢(jian)測(ce)(ce)(ce)(ce)區域進行照射(she)時,照射(she)區域內大(da)(da)腸桿菌滅殺(sha)率大(da)(da)于99%,霉菌滅殺(sha)率大(da)(da)于95%(朗普(pu)(pu)(pu)實驗測(ce)(ce)(ce)(ce)試(shi)數據(ju),僅供參考(kao)),符合國家消(xiao)(xiao)毒技術規范。朗普(pu)(pu)(pu)科技專(zhuan)(zhuan)(zhuan)注(zhu)殺(sha)菌領域十一年,只做有效殺(sha)菌!我(wo)們將(jiang)為您提供技術支持,專(zhuan)(zhuan)(zhuan)業選(xuan)型。抗擊疫(yi)情(qing)(qing),我(wo)們在行動!
本文參考出處:
1.國家衛健委關于(yu)印發(fa)新型冠狀病毒肺炎(試行第五、六、七(qi)版)的通知;
2.參(can)考文(wen)獻:UV irradiation and autoclave treatment for elimination of contaminating DNA from laboratory consumables. 《Forensic Science International Genetics》, 2010,4(2) :89